Detección de receptores de estrógeno, progesterona y de proteína ligadora de corticosteroides en el tracto genital de hembras caninas (Cannis familiaris): estudio inmunohistoquímico / Detection of estrogen, progestorone receptors and corticosteroid binding globulin in the canine (Cannis familiaris) reproductive female tract: immunohistochemistry study

Los receptores esteroidales sexuales del tracto genital de la hembra tienen importancia dado que, a través de ellos, actúan las hormonas responsables de su desarrollo y de sus cambios morfofuncionales. En su mecanismo, uno de los factores a considerar son las posibles diferencias entre las distintas especies. El objetivo del presente estudio fue evaluar, en la especie canina (N=8), la presencia de receptores de estrógenos (RE), progesterona (RP) y la presencia de proteína ligadora de corticosteroides (CBG) en ovario, oviducto y útero de 3 hembras prepúberes (N=3) y 5 hembras adultas (N=5). La evaluación morfológica se realizó con tinción de hematoxilina-eosina (H-E) e inmunocitoquímica, según la técnica de Stemberger (1979). Los resultados revelaron en animales ciclando, inmunorreactividad (IR) positiva, RE en útero, oviducto y ovario siendo marcada en oviducto. La IR para RP fue leve y varió según el estadio del ciclo. En hembras prepúberes, los RE y RP no fueron evidenciados. La CBG mostró positividad en el tracto durante el ciclo y fue negativa en prepúberes. Se concluye que la presencia de RE y RP es detectable en útero, oviducto y ovario de la hembra canina adulta habiendo variaciones de concentración según el estadio del ciclo estral, siendo su presencia en las prepúberes no evidenciables, a diferencia de otras especies (oveja), donde son detectables en este estadio del desarrollo. La presencia de CBG constante durante el ciclo estral y variable en los otros estadios indica su posible participación en los procesos reproductivos.

The sexual steroid receptors of the genital tract of the female have significant importance since though them the hormones responsible acting. In their mechanism one of the factors to be kept in mind is the possible differences among the diverse species, the objective of this study was to evaluate in canine (N= 8) the presence of receptors of estrogens (ER), progesterone (PR) and the presence of corticosteroid binding globuline (CBG) in ovaries, oviduct and uterus of prepuberal female (N=.3) and matures: (N=5) It was used H-E and immunohistochemical study according to the technique of Stenberger (1979). The results during the oestrus showed: Immune reactivity (IR) positive, ER in uterus and ovary being much stronger in oviduct. The PR varied according the stage of oestrus cycle. In puberal female dog ER and PR were undetectable. The CBG revealed positive in reproductive tract of cycling females but negative in prepubertal. It was concluded that the presence of ER and PR are detectable in uterus, oviduct and ovary of mature female dogs varying their concentration according to the cycle. In prepuberal, its presences come undetectable differing from other species (ovine), in which they are detected at this stage of development. The constant of CBG presence during oeustrus cycle and such variation in other stages of the cycle, it might suggest its participation in the several reproductive activities.

Are there inflammatory changes in the hepatic biopsies of patients with acute cholangitis of lithiasic origin?

INTRODUCTION: Acute cholangitis (AC) is a clinical diagnosis entity. A high prevalence of acute inflammatory changes (AIC) are found in hepatic needle biopsies (HB) in these patients. The aim of our study is to determine the prevalence of AIC in HB performed on patients with AC due to biliary stones. METHODS: Cross-sectional study. Surgery was performed at the Hospital Regional of Temuco. Patients with Charcot's triad, over 15 years of age, and surgical exploration of common bile duct were included. There was exclusion if periampullary tumor or liver cirrhosis was suspected. Bile macroscopy and hepatic histopathology were determined. A hepatic needle biopsy was taken during surgery. Biopsies were analyzed by two independent pathologist groups and kappa statistics were applied. RESULTS: From January to June, 2002, fifty-three patients (83% female) with a median age of 60.7 years with AC were operated on. We found 83% with pus in the biliary system and 17% with cloudy bile. Histopathology: minimal changes (32%), hepatic steatosis (25%), chronic periportal infiltration (17%), liver cirrhosis (11%), AIC (6%) and normal (9%). Kappa statistics were 0.77. In conclusion a low prevalence of AIC was found in the HB of patients with AC.

Presencia de cbg (corticosteroid binding globulin) y receptores de estrógeno (fracción alfa) y progesterona en el sistema reproductor de ovejas en distintos estadios del ciclo reproductivo. Estudio inmunohistoquímico / Presence of CBG (Corticosteroid binding globulin) Estrogen Receptors Alpha and Progesterone Receptors Along the Reproductive Tract in Sheep During Different Stages of the Reproductive Cycle. Inmunohistochemestry Study

Las hormonas esteroidales sexuales actúan a través de sus receptores permitiendo el desarrollo del tracto genital y determinando su estado morfofuncional. El objetivo del presente estudio fue estudiar la presencia de CBG (corticosteroid binding globulin) y de los receptores de estrógenos alfa y progesterona en el útero, oviducto y ovario de ovejas cíclicas (n = 3), gestantes (n = 3), y en anestro (n = 3). La evaluación morfológica se realizó con tinción de hematoxilina - eosina (H-E) e inmunohistoquímica, según técnica de Sternberger. Los resultados revelaron para receptores de estrógenos en oveja cíclicas, inmunorreactividad positiva moderada en el epitelio glandular y estroma endometrial y en epitelio superficial y corión de la mucosa oviductal; siendo la inmunorreactividad leve en ovejas gestantes y anéstricas. Los receptores de progesterona mostraron en los 3 órganos inmunorreactividad positiva moderada en ovejas cíclicas e inmunorreactividad leve o negativa en animales acíclicos. La inmunorreactividad para CBG en endometrio fue marcada en ovejas cíclicas, y leves o negativa en gestantes y anéstricas. En ovario se observó en ovejas durante el ciclo, reacción inmunopositiva a la CBG en células foliculares, en el fluido folicular y en células estromales llenas de material granular inmunopositivo dispuestas en forma grupal o aislada. Se concluye que la presencia de los receptores de estrógenos alfa y de progesterona es siempre detectable en útero, oviducto y ovario variando su concentración según el estadio. La presencia de CBG fue constante en los tres órganos durante el ciclo estral y variable en los otros estadios, siendo su participación dentro del proceso reproductivo tema de próximos estudios.

The steroids hormones operate through their receptors in the development of the genital tract and determine it morphofuncional state. The objective of the present study was to investigate the presence of CBG (corticosteroid binding globulin), estrogens alpha and progesterone receptors in the uterus, oviduct and ovary of sheep during oestrous cycle (n = 3), pregnancy (n = 3), and in anoestrous (n = 3). Cross sections were stained with Hematoxylin-eosin and Inmunohistochemical (ICQ) study was made according to the technique of Sternberger. The results showed for estrogens receptors in sheep on cycle estral, moderate positive immunoreaction in the estroma and on the epithelial cells of endometrial glands and in oviductal superficial epithelium and estroma; the immunoreaction in pregnant and anestrics sheep was low. The progesterone receptors had in the 3 organs moderate immunorreactivity in cycling sheep and immunorreactivity low or negative in acycling animals. The CBG endometrial immunorreaction was high in cycling sheep and low or negative in pregnant and anestrics. The CBG in sheep during the cycle demonstrated a positive reaction at ovarian level in follicular cells, inside follicular liquid, and in stromal cells filled with granulate material disseminated in groups or isolated. In conclusion, the presence of estrogens alpha and progesterone receptors are always detectable in uterus, oviduct and ovary varying their concentration according to the stage The presence of CBG was constant in the three organs during the oestral cycle and variable in the other stages, its participation in the reproductive process is topic for next studies.